Selenoprotein S protects against adipocyte death through mediation of the IRE1α-sXBP1 pathway.

TitleSelenoprotein S protects against adipocyte death through mediation of the IRE1α-sXBP1 pathway.
Publication TypeJournal Article
Year of Publication2018
AuthorsL Men, S Yu, J Yao, Y Li, D Ren, and J Du
JournalBiochemical and biophysical research communications
Volume503
Issue4
Start Page2866
Pagination2866 - 2871
Date Published09/2018
Abstract

As the most conserved branch of the unfolded protein response (UPR), the inositol-requiring enzyme 1a (IRE1a)/X-box binding protein 1 (XBP1) pathway plays crucial roles in cell survival and cell death by upregulating UPR-associated genes involved in protein entry into the endoplasmic reticulum (ER) and ER-associated degradation (ERAD). Selenoprotein S (SelS) is localized to the ER membrane and involved in ERAD. Although SelS plays an important role in restoring ER stress, the SelS-dependent protective mechanisms against cell death remain unclear. Here, using an inducible SelS knockdown (KD) 3T3-L1 cell model, we showed that SelS KD resulted adipocyte death, which was associated with imbalance of the Bcl-2 family members. Furthermore, SelS KD decreased spliced XBP1 (sXBP1), increased IRE1α and p-JNK, suggesting a role of SelS in the modulation of the IRE1α-sXBP1 pathway. Moreover, adipocyte death induced by SelS suppression can be inhibited by overexpression of sXBP1. Thus, it is proposed that SelS promotes cell survival through the IRE1α-XBP1 signaling pathway.

DOI10.1016/j.bbrc.2018.08.057
Short TitleBiochemical and biophysical research communications