| Title | High-speed label-free functional photoacoustic microscopy of mouse brain in action. |
| Publication Type | Journal Article |
| Year of Publication | 2015 |
| Authors | J Yao, L Wang, J-M Yang, KI Maslov, TTW Wong, L Li, C-H Huang, J Zou, and LV Wang |
| Journal | Nature methods |
| Volume | 12 |
| Issue | 5 |
| Start Page | 407 |
| Pagination | 407 - 410 |
| Date Published | 05/2015 |
| Abstract | We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain. |
| DOI | 10.1038/nmeth.3336 |
| Short Title | Nature methods |