Achievements of PAT in videos

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  1. Dr. Lihong Wang's 's Hot Topics Presentation from SPIE Photonics Europe 2012.
  2. Photoacoustic microscopy of small intestine in a mouse in vivo
    Double illumination photoacoustic microscopy of the dense vasculature in the small intestine of a mouse in vivo after a major desection surgery. This was a project collaborating with Dr. Brad Warner, Washington University School of Medicine.
    Reference: Yao, Junjie, Konstantin I. Maslov, Ernest R. Puckett, Kathryn J. Rowland, Brad W. Warner, and Lihong V. Wang. "Double-illumination photoacoustic microscopy." Optics letters 37, no. 4 (2012): 659-661.

  3. PAM of cerebral responses to electrical stimulations to the hindlimbs.
    A 3×4 mm2 cortical area covering the somatosensory regions of both hemispheres was imaged at a volumetric rate of 1 Hz. Fractional PA amplitude changes (shown in yellow), in response to the left hindlimb stimulation (LHS) and right hindlimb stimulation (RHS), were superimposed on the vascular image (shown in red). The curve at the bottom of the video shows the average PA signal amplitude in the somatosensory regions.

    Reference: Yao, Junjie, Lidai Wang, Joon-Mo Yang, Konstantin I. Maslov, Terence TW Wong, Lei Li, Chih-Hsien Huang, Jun Zou, and Lihong V. Wang. "High-speed label-free functional photoacoustic microscopy of mouse brain in action." Nature methods 12, no. 5 (2015): 407-410.

  4. High-speed photoacoustic microscopy of the blood flow in a mouse ear in vivo.
    A pair of artery and vein can be clearly imaged with the red blood cells flowing at speeds up to 1 cm/s. The field of view is 1 mm by 0.5 mm, with a volumetric imaging frame rate of 2 Hz.

    References: J Yao, CH Huang, L Wang, JM Yang, L Gao, KI Maslov, J Zou, LV Wang, Journal of biomedical optics 17 (8), 0805051-0805053

  5. Water-immersible MEMS scanning mirror for PAM
    This small mirror, fabricated by Dr. Jun Zou (Texas A&M), can perform fast scanning (up to 1 kHz frame rate) of both optical and acoustic beams, while fully immersed in water. This mirror is an essential component for our fast-scanning PAM system. In this video, we demo the fast scanning of the laser beam by the MEMS mirror. To enhance the visibility of the laser beam, we stirred the water with a green marker pen to make the water fluorescent.

    Reference: Huang, Chih-Hsien, Junjie Yao, Lihong V. Wang, and Jun Zou. "A water-immersible 2-axis scanning mirror microsystem for ultrasound andha photoacoustic microscopic imaging applications." Microsystem technologies 19, no. 4 (2013): 577-582.

  6. Whole-body photoacoustic computed tomography of mouse internal
    organs in vivo.
    The light fluence on the animal skin was 8 mJ/cm2 at 780 nm. Stepping along the animal trunk with a step size of 0.3 mm, a total of 250 cross-sectional images were acquired with a field of view of 2 cm by 3 cm.
    Reference: Yao, Junjie, Andrii A. Kaberniuk, Lei Li, Daria M. Shcherbakova, Ruiying Zhang, Lidai Wang, Guo Li, Vladislav V. Verkhusha, and Lihong V. Wang. "Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe." Nature methods 13, no. 1 (2016): 67-73.

  7. Reversible-switchable PACT of a tumor in a nude mouse
    Elevational-scanning whole-body photoacoustic computed tomography (PACT) of a BphP1-expressing U87 tumor in vivo with a scanning step size of 1 mm. The differential PA signals from the tumor (shown in color) were superimposed on top of the OFF state PA signals from blood (shown in gray). A global threshold was applied to all the differential images with a threshold level at three times the noise level.
    Reference: Yao, Junjie, Andrii A. Kaberniuk, Lei Li, Daria M. Shcherbakova, Ruiying Zhang, Lidai Wang, Guo Li, Vladislav V. Verkhusha, and Lihong V. Wang. "Multiscale photoacoustic tomography using reversibly switchable bacterial phytochrome as a near-infrared photochromic probe." Nature methods 13, no. 1 (2016): 67-73.
  8. Deep penetration photoacoustic microscopy of implants in pig skin
    This ex vivo pig skin sample was imaged by our acoustic-resolution photoacoustic microscopy, with a penetration of more than 5 mm. The implant is colorcoded in purple and the blood vessels are in yellow. This is a good example of the superior penetration of photoacoustic imaging over traditional optical microscopy.