Photoacoustic imaging using genetically encoded reporters: a review.

TitlePhotoacoustic imaging using genetically encoded reporters: a review.
Publication TypeJournal Article
Year of Publication2017
AuthorsJ Brunker, J Yao, J Laufer, and SE Bohndiek
JournalJournal of biomedical optics
Volume22
Issue7
Date Published07/2017
Abstract

Genetically encoded contrast in photoacoustic imaging (PAI) is complementary to the intrinsic contrast provided by endogenous absorbing chromophores such as hemoglobin. The use of reporter genes expressing absorbing proteins opens the possibility of visualizing dynamic cellular and molecular processes. This is an enticing prospect but brings with it challenges and limitations associated with generating and detecting different types of reporters. The purpose of this review is to compare existing PAI reporters and signal detection strategies, thereby offering a practical guide, particularly for the nonbiologist, to choosing the most appropriate reporter for maximum sensitivity in the biological and technological system of interest.

DOI10.1117/1.jbo.22.7.070901
Short TitleJournal of biomedical optics