Title | High-speed widefield photoacoustic microscopy of small-animal hemodynamics. |
Publication Type | Journal Article |
Year of Publication | 2018 |
Authors | B Lan, W Liu, Y-C Wang, J Shi, Y Li, S Xu, H Sheng, Q Zhou, J Zou, U Hoffmann, W Yang, and J Yao |
Journal | Biomed Opt Express |
Volume | 9 |
Issue | 10 |
Start Page | 4689 |
Pagination | 4689 - 4701 |
Date Published | 10/2018 |
Abstract | Optical-resolution photoacoustic microscopy (OR-PAM) has become a popular tool in small-animal hemodynamic studies. However, previous OR-PAM techniques variously lacked a high imaging speed and/or a large field of view, impeding the study of highly dynamic physiologic and pathophysiologic processes over a large region of interest. Here we report a high-speed OR-PAM system with an ultra-wide field of view, enabled by an innovative water-immersible hexagon-mirror scanner. By driving the hexagon-mirror scanner with a high-precision DC motor, the new OR-PAM has achieved a cross-sectional frame rate of 900 Hz over a 12-mm scanning range, which is 3900 times faster than our previous motor-scanner-based system and 10 times faster than the MEMS-scanner-based system. Using this hexagon-scanner-based OR-PAM system, we have imaged epinephrine-induced vasoconstriction in the whole mouse ear and vascular reperfusion after ischemic stroke in the mouse cortex in vivo, with a high spatial resolution and high volumetric imaging speed. We expect that the hexagon-scanner-based OR-PAM system will become a powerful tool for small animal imaging where the hemodynamic responses over a large field of view are of interest. |
DOI | 10.1364/BOE.9.004689 |
Short Title | Biomed Opt Express |