Title | Near-infrared optical-resolution photoacoustic microscopy. |
Publication Type | Journal Article |
Year of Publication | 2014 |
Authors | P Hai, J Yao, KI Maslov, Y Zhou, and LV Wang |
Journal | Optics letters |
Volume | 39 |
Issue | 17 |
Start Page | 5192 |
Pagination | 5192 - 5195 |
Date Published | 09/2014 |
Abstract | Compared with visible light (380-700 nm), near-infrared light (700-1400 nm) undergoes weaker optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared optical-resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using optical fluence within the American National Standards Institute (ANSI) limit (100 mJ/cm2). Beyond ∼0.6 mm deep in chicken breast tissue, NIR-OR-PAM has shown finer resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well. |
DOI | 10.1364/ol.39.005192 |
Short Title | Optics letters |