|Title||Near-infrared optical-resolution photoacoustic microscopy.|
|Publication Type||Journal Article|
|Year of Publication||2014|
|Authors||P Hai, J Yao, KI Maslov, Y Zhou, and LV Wang|
|Pagination||5192 - 5195|
Compared with visible light (380-700 nm), near-infrared light (700-1400 nm) undergoes weaker optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared optical-resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using optical fluence within the American National Standards Institute (ANSI) limit (100 mJ/cm2). Beyond ∼0.6 mm deep in chicken breast tissue, NIR-OR-PAM has shown finer resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well.
|Short Title||Optics Letters|