Near-infrared optical-resolution photoacoustic microscopy.

TitleNear-infrared optical-resolution photoacoustic microscopy.
Publication TypeJournal Article
Year of Publication2014
AuthorsP Hai, J Yao, KI Maslov, Y Zhou, and LV Wang
JournalOptics Letters
Volume39
Issue17
Start Page5192
Pagination5192 - 5195
Date Published09/2014
Abstract

Compared with visible light (380-700 nm), near-infrared light (700-1400 nm) undergoes weaker optical attenuation in biological tissue; thus, it can penetrate deeper. Herein, we demonstrate near-infrared optical-resolution photoacoustic microscopy (NIR-OR-PAM) with 1046 nm illumination. A penetration depth of 3.2 mm was achieved in chicken breast tissue ex vivo using optical fluence within the American National Standards Institute (ANSI) limit (100  mJ/cm2). Beyond ∼0.6  mm deep in chicken breast tissue, NIR-OR-PAM has shown finer resolution than the visible counterpart with 570 nm illumination. The deep imaging capability of NIR-OR-PAM was validated in both a mouse ear and a mouse brain. NIR-OR-PAM of possible lipid contrast was explored as well.

DOI10.1364/ol.39.005192
Short TitleOptics Letters